Antibacterial Activity Of Plant Extracts By Well Diffusion Method


The test organisms were inoculated in nutrient broth and incubated overnight at 37°c to adjust the turbidity to 0.5 mcfarland standards giving a final inoculum of 1.5 × 108 cfu/ml. Accordingly, the diffusion method is commonly applied to screen the antimicrobial activity of the plant bioactive compounds.

(a) Antibacterial activity of biosynthesized AgNPs using agar well
(a) Antibacterial activity of biosynthesized AgNPs using agar well from www.researchgate.net

The leaves and flowers were extracted consecutively by solvents of different polarities such as hexane, chloroform, ethyl acetate, and methanol. Three solvents with different polarities were used to extract antimicrobial agents from the plants via maceration technique.

Antibacterial Activity Of Plant Extracts By Well Diffusion Method. Diffuser is a device that is commonly owned by aromatherapy lovers. There are many types and uses of diffusers available depending on the requirements of users. Make sure you are right in selecting one that is suitable for you so that the benefits of aromatherapy can be experienced to the fullest extent.

A diffuser is a piece of equipment that functions to convert aroma oils in vapors, then spread them in the air to ensure they are more comfortable to breathe. The use of a diffuser in the room can create a comfortable and calming ambience. Therefore, the use of diffusers is a great way to calm the mind and ease anxiety.

While the diffusion of air has several benefits It isn't safe to make use of it for making antiseptic liquids vaporize to sterilize the air. This actually risks causing side consequences, including irritation of the respiratory tract as well as the eyes.

The use of a diffuser so far has only been effective in maximising the benefits that essential oils provide. The aroma that is diffused can stimulate the senses and influences emotions as well as your nervous system. However, it depends on the type of aromatherapy
A diffuser is a tool that spreads something over a large area. In this instance the items being spread are essential oils and diffusers as instruments.

The diffuser is made to treat essential oil droplets with water to create a fine steam, which is then diffused throughout the space. The resulting steam usually is a soothing aroma and is beneficial to well-being.

Research has shown that in general, the role of a diffuser is to give a therapeutic effect or healing result as it releases particles made from essential oils. The particles trigger the nerve system and transmit signalling to the limbic systems within the brain.

The brain responds by performing various physiological functions for example, releases of hormones reducing pain, or a positive improvement in mood.

Other benefits of a diffuser include the treatment of respiratory problems, mood enhancement as well as relaxing, air purification and much more, based what essential oils are used.

The best way to use the diffuser is 30 to 60 minutes, by dripping 3 drops essential oils. In addition, make sure the area where the diffuser will be used has good ventilation.

To function at its best it is recommended that the diffuser be placed in a strategic location, like the desk at work or next to the bed, or in the right of the living room.


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